異檸檬酸脱氫酶 (NADP+)

異檸檬酸脱氫酶 (NADP+)
大肠杆菌（E. coli）異檸檬酸脱氫酶(NADP+)的晶体结构（PDB 1CW7） 这种酶有三个活性位点，三分子柠檬酸中的其中一个在NADP+结合区域中。
命名 系统命名 isocitrate:NADP+ oxidoreductase (decarboxylating) 缩写 IDP; IDP1; IDP2; IDP3; IDH（有歧义） 别名 oxalosuccinate decarboxylase; isocitrate dehydrogenase (NADP); oxalsuccinic decarboxylase; isocitrate (NADP) dehydrogenase; isocitrate (nicotinamide adenine dinucleotide phosphate) dehydrogenase; NADP-specific isocitrate dehydrogenase; NADP-linked isocitrate dehydrogenase; NADP-dependent isocitrate dehydrogenase; NADP isocitric dehydrogenase; isocitrate dehydrogenase (NADP-dependent); NADP-dependent isocitric dehydrogenase; triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase; NADP+-linked isocitrate dehydrogenase; dual-cofactor-specific isocitrate dehydrogenase; NADP+-ICDH; NADP+-IDH
识别码
EC編號	1.1.1.42
CAS号	9028-48-2
数据库
IntEnz	IntEnz浏览
BRENDA	BRENDA入口
ExPASy	NiceZyme浏览
KEGG	KEGG入口
MetaCyc	代谢路径
PRIAM	概述
PDB	RCSB PDB PDBj PDBe PDBsum
基因本体	AmiGO / EGO
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異檸檬酸脱氫酶 (NADP⁺)（英語：isocitrate dehydrogenase (NADP⁺)，EC 1.1.1.42 （页面存档备份，存于互联网档案馆））是一种以NAD⁺或NADP⁺为受体、作用于供体CH-OH基团上的氧化还原酶。这种酶能催化以下酶促反应：

異檸檬酸 + NADP⁺ $\rightleftharpoons$ 2-酮戊二酸 + CO₂ + NADPH

異檸檬酸脱氫酶 (NADP⁺)需要Mn²⁺或Mg²⁺以维持活性。真核细胞中的异柠檬酸脱氢酶 (NAD⁺)以两种形式存在：其中一种与NADP⁺还存在于细胞质基质中不能变构调节；另一种与NAD⁺相关联且仅在线粒体中被发现并但可以变构调节。 这两种酶——异柠檬酸脱氢酶 (NADP⁺)与异柠檬酸脱氢酶 (NAD⁺)并不相同，前者能以草酰琥珀酸作为底物。某些物种的异柠檬酸脱氢酶 (NADP⁺)也能利用NAD⁺，但催化的反应速率较慢。

参考文献

• Agosin, M.U. and Weinbach, E.C. Partial purification and characterization of the isocitric dehydrogenase from ‘’Trypanosoma cruzi’’.. Biochim. Biophys. Acta. 1956, 21: 117–126. PMID 13363868 （英语）. 
• Moyle, J. and Dixon, M. Purification of the isocitrate enzyme (triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase).. Biochem. J. 1956, 63: 548–552. PMID 13355848 （英语）. 
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• Koh, H.J., Lee, S.M., Son, B.G., Lee, S.H., Ryoo, Z.Y., Chang, K.T., Park, J.W., Park, D.C., Song, B.J., Veech, R.L., Song, H. and Huh, T.L. Cytosolic NADP⁺-dependent isocitrate dehydrogenase plays a key role in lipid metabolism.. 2004: 39968–39974. PMID 15254034 （英语）. 
• Ceccarelli, C., Grodsky, N.B., Ariyaratne, N., Colman, R.F. and Bahnson, B.J. Crystal structure of porcine mitochondrial NADP⁺-dependent isocitrate dehydrogenase complexed with Mn²⁺ and isocitrate. Insights into the enzyme mechanism.. J. Biol. Chem. 277. 2002: 43454–43462. PMID 12207025 （英语）.